Three RPLCxRPLC systems were defined analytical geometry of two dimensions pdf successfully used to detect pharmaceutical impurities. Both degree of orthogonality and practical peak capacity were used to find out suitable 2D-conditions.
About 1000 as effective peak capacity could be achieved in less than 50 min in RPLC x RPLC. MS detection was highlighted with two real pharmaceutical samples. One or two appropriate RPLCxRPLC systems are sufficient for impurity analysis in the course of drug development. A comparative study of different sets of chromatographic conditions including stationary phase, pH additive and organic modifier was carried out with two real pharmaceutical samples in order to find out the best analytical conditions for implementation of one or several generic on-line LCxLC separations.
Our choice was based on the evaluation of both degree of orthogonality and practical sample peak capacity under linear gradient conditions. The potential of 190 combinations of chromatographic systems was compared. A set of 3 RPLCxRPLC configurations was found to be very attractive for both samples and in good agreement with the findings of a previous study carried out with 17 model compounds, thereby supporting the idea of using generic LCxLC conditions in the pharmaceutical area. MS analysis of two pharmaceutical samples. It was shown, for each sample, that these 2D-systems were able to generate an effective peak capacity close to 1000 in less than 50 min.